Fig. 1: Quantitative Mutational Scan sequencing.
From: High-throughput method characterizes hundreds of previously unknown antibiotic resistance mutations
A Experimental technique. The 24-h mutant accumulation step uses 32 subpopulations to reduce the final population’s bias towards mutants that by chance arise earlier. B Sequencing and variant calling pipeline. C Experimental design. We tested three antibiotics and four strains of E. coli, performing three replicates for each of the 12 conditions, comprising 36 independent samples. The wild type (WT) strain is BW25113, which was the ancestor of the three rifampicin-resistant strains with mutations in rpoB. One nitrofurantoin/Q513R replicate was excluded from the analysis due to sequencing failure.
