Fig. 5: Case studies of notable resistance mutation targets.
From: High-throughput method characterizes hundreds of previously unknown antibiotic resistance mutations
A Three genes that were targeted by high-occurrence mutations in their annotated regulatory sequences59,60, including one with a start codon mutation. B Genes with the highest occurrence of synonymous mutations. These mutations were all in the same codon, swapping Ile-ATC to Ile-ATA, even though nearby Ile-ATT or -ATA codons were not targeted. Some of the genes were also targeted by non-synonymous mutations (downward arrows). C Cysteine tRNA aminoacylation (where cysT is charged with the cysteine amino acid by tRNA synthetase). We observed ciprofloxacin-specific mutations in cysT, which, in principle, should prevent its interaction with cysteine.
