Fig. 5: CHD6 DNA binding activity is essential for ATP-dependent nucleosome remodeling. | Nature Communications

Fig. 5: CHD6 DNA binding activity is essential for ATP-dependent nucleosome remodeling.

From: CHD6 has poly(ADP-ribose)- and DNA-binding domains and regulates PARP1/2-trapping inhibitor sensitivity via abasic site repair

Fig. 5

For all statistical analysis in this figure, 2-way ANOVAs followed by Tukey post hoc tests were carried out. ns = not significant (p > 0.05), *p < 0.05. ** p < 0.01. ***p < 0.001.****p < 0.0001. Source data are provided as a Source Data file. Panel a. Schematic of wildtype and point mutants of hCHD6 aa 270-1618, as well as the hCHD6 aa270-1029 truncation mutant lacking both DBD1 and DBD2 entirely. Panel b Ability of indicated proteins from (a) to bind mononucleosomes (147 bp DNA around the histone octamer and 50 bp linker DNA) was assessed by EMSA. Panel c ATP hydrolysis assay with proteins from (a) in presence or absence of free double-stranded DNA (dsDNA) or mononucleosomes from (b); centre lines of ATP assay data represent arithmetic means ± SD; n = 3. Panel d. Workflow for chromatin remodeling assay; this figure was created with Biorender.com (agreement number UO268HP0OZ) and Microsoft PowerPoint. Panel e–g Wildtype CHD6 and indicated mutants (from a) were assayed with and without ATP using chromatin remodeling assay outlined in (d). All FRET data line graphs are geometric means ± CI95%; n = 3.

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