Fig. 9: CHD6-deletion causes increased Fpg-enzyme sensitive base damage persistence following H2O2 exposure. | Nature Communications

Fig. 9: CHD6-deletion causes increased Fpg-enzyme sensitive base damage persistence following H2O2 exposure.

From: CHD6 has poly(ADP-ribose)- and DNA-binding domains and regulates PARP1/2-trapping inhibitor sensitivity via abasic site repair

Fig. 9

For all statistical analysis in this figure, 2-way ANOVAs followed by Tukey post hoc tests were carried out. ns = not significant (p > 0.05), *p < 0.05. ** p < 0.01. ***p < 0.001.****p < 0.0001. Source data are provided as a Source Data file. Panel a A workflow for Fpg-modified and standard alkaline comet assay; this figure was created using Microsoft PowerPoint. Panel b. WT and RPE-1ΔCHD6 in suspension were treated with 50 µM H2O2 and analyzed by Fpg-modified and standard alkaline comet assay, with scatters representing raw data from three independent experimental repeats and centre lines representing geometric means ± CI95%; n = 3. Panel c Geometric means and 95% confidence intervals from (b) were expressed as a bar chart representing, showing the differences between standard and Fpg-modified comet assay tail moments as numbers in parentheses above each time point.

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