Fig. 1: Connecting the cores, subtelomeres and bloodstream VSG expression sites of the T. brucei megabase chromosomes through Nanopore long-read sequencing. | Nature Communications

Fig. 1: Connecting the cores, subtelomeres and bloodstream VSG expression sites of the T. brucei megabase chromosomes through Nanopore long-read sequencing.

From: Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei

Fig. 1

A Circos plots highlighting synteny between the Muller genome assembly and Nanopore contigs (tigs) of two megabases chromosomes (4 and 5). Grey ribbons represent overlaps, black ribbons represent multiple overlaps within a region, red denotes VSG genes, and blue denotes a centromere; for clarity of comparison, the organisation of the transcribed core, transcriptionally silent subtelomeres (numbered 3 A, 3B, 5 A, 5B) and bloodstream VSG expression sites (BESs) in these chromosomes in the Muller genome assembly are diagrammed in the lower panel (adapted from9). B Distinct behaviour of the core and subtelomere compartments of chromosomes 4 and 5 Nanopore contigs is shown by mapping of R-loops (DNA-RNA hybrid immunoprecipitation and sequencing, DRIP; data shown as IP/input) in wild type (WT) bloodstream from cells and RNase H1 null (-/-) mutants, as well as by mapping of RNA-seq data from WT cells (RPKM: reads per Kb per million mapped reads). Elements of panel A were created in BioRender. McCulloch, R. (2025) https://BioRender.com/u96k590.

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