Fig. 5: Assembly of 177 bp-containing sub-megabase T. brucei chromosomes that harbour diverse transcribed genes. | Nature Communications

Fig. 5: Assembly of 177 bp-containing sub-megabase T. brucei chromosomes that harbour diverse transcribed genes.

From: Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei

Fig. 5

A A diagrammatic representation of two distinct forms of Nanopore contigs, one type housing a telomeric VSG BES (orange) and each containing 177 bp repeats characteristic of sub-megabase chromosomes of T. brucei. B RNA-seq mapping to eight sub-megabase chromosome Nanopore contigs (tigs). C Circos plots showing correspondence between the sub-megabase chromosome Nanopore contigs and many previously unassigned unitigs; grey ribbons represent simple overlaps, and black ribbons represent multiple overlaps within a region. D Gene content of the novel, smaller chromosomes: only the most abundant predicted gene types are detailed (total numbers are shown), amongst which the range of orthology groups (OG6) predicted amongst hypothetical genes by OrthoMCL is illustrated. Elements of panel A were created in BioRender. McCulloch, R. (2025) https://BioRender.com/i58h117.

Back to article page