Fig. 2: Identification of cerebral blood flow (CBF) regulation-related genes in microglia.
From: Microglia modulate the cerebrovascular reactivity through ectonucleotidase CD39
a Schematic of bulk RNA sequencing with mice brain cortex tissue from three different treatment groups (baseline, PLX, repopulation). Five mice were included in each group (Created in BioRender. Lab, K. (2025) https://BioRender.com/r63x287). b Principal component analysis (PCA) plots outlining the similarities between samples. c Volcano plot of the cortex genes showing the magnitude (log2 [fold change]) and probability ( − log10 [adjusted p-value]) in the PLX3392-treated group versus the baseline group. Black and gray dots represent significantly changed and non-significantly changed genes, respectively. d Heatmap showing the expression of selected genes in baseline, PLX3397-treated, and repopulated mouse brains based on the row z scores of log2 CPM values. e RT-qPCR verification of the interested genes’ expression in three conditions: baseline, PLX3397-treated and repopulation. Data are mean ± s.e.m. Each dot represents one mouse. n = 5 mice for each group. p-values were determined by one-way ANOVA with the Tukey post hoc test. Source data are provided as a Source Data file. f, g Representative images of multi-color RNAscope experiments showing CD39 (Entpd1) expression in microglia (Hexb+) (f) and endothelial cells (Pecam1+) (g), respectively. Top, bar = 100 μm; bottom, bar = 50 μm. Each experiment was repeated independently for four times with similar results.
