Fig. 7: Microbiota-derived spermidine promotes browning of mouse white adipose tissue and 3t3-L1 adipocytes.
From: Lactobacillus reuteri ZJ617 attenuates metabolic syndrome via microbiota-derived spermidine
a Representative infrared thermal images and b the mean temperatures in WAT were measured at the end of the experiment (n = 9 per group). c Frequency distribution of adipocyte sizes. d Representative H&E picture of WAT (scale bars, 100 μm). e WAT weights (n = 9 per group). f Representative TEM images of WAT (scale bars, 0.5 μm). g Protein levels of ADRB3 and UCP1 in WAT (n = 6 per group). Samples were derived from the same experiment, and gels/blots were processed in parallel. h mRNA levels of non-shivering thermogenesis-related genes relative to Gapdh in WAT (n = 6 biological replicates per group). i Spermidine contents in WAT (HFD, HFD + Spermidine, HFD + ZJ617 + DA, n = 9, HFD + ZJ617, n = 8). j Representative images showing the status of lipid droplets stained with Bodipy 493/503 (green) or Oil red O staining in 3T3-L1 adipocyte (scale bars, 100 μm). k Protein levels of ADRB3 and UCP1 in 3T3-L1 adipocyte (n = 3 per group). The 3T3-L1 adipocyte was independently cultured three times. Data were shown as mean ± SD. Significances in all panels are calculated based on one-way ANOVA with Å Ãdák for multiple-group comparisons, with the exception of (k) calculated based on two-tailed t-tests. Source data are provided as a Source Data file.
