Fig. 1: Single-cell RNA-seq and cell trajectory analysis delineate the lineage hierarchy of CD26⁺ mesenchymal stromal cells.

a UMAP plot and keys to the clustering and cell types are shown to the right. MSC, mesenchymal stromal cell. b Bubble diagram of key markers of each cluster. c UMAP plot of re-clustering of MSC and tenocyte in 1 a and representative markers (Cd26, Ly6a, Cd90, and Scx). d Violin map of key genes of CD26⁺ MSCs, Apoe⁺ progenitors, Sema5a⁺ progenitors. e CytoTRACE analysis of CD26⁺ MSCs, Apoe⁺ progenitors, Sema5a⁺ progenitors. f Heatmap of differentially expressed genes ordered based on their common kinetics through pseudotime displayed at each trajectory branch point as defined in the Monocle trajectory analysis of the CD26⁺ MSCs, Apoe⁺ progenitors and Sema5a⁺ progenitors.