Fig. 5: IFNλ3 and IFNλ4 exhibit kinetic differences in antiviral gene induction, leading to differential antiviral activity in vitro.
From: Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering
A pSTAT1 signaling of IFNω1 (orange), IFNλ3 (blue), and IFNλ4 (red) in Hap1 cells. Curves are fit to a first-order logistic model. Data are presented with mean values and error bars representing ±SEM (n = 3 biologically independent experiments). B Relative quantification (RQ) of select genes induced by IFNω1, IFNλ3, and IFNλ4 in Hap1 cells treated with saturating concentrations (100 nM) interferon for 6 h as measured by qPCR. Data are presented with mean values. Error bars represent 95% confidence intervals (n = 3 biologically independent experiments). C Changes in induction of ISG15 or MX1 over time for IFNλ3 and IFNλ4 in Huh7.5.1 cells at a range of concentrations (~0.5, 1.5, 5, and 15 nM). Statistical significance determined by a two-tailed student t-test. Data are presented as mean values ± SD (n = 3 biologically independent experiments; * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001). D Intracellular HCV genomic RNA level over time following treatment with IFNω1, IFNλ3, or IFNλ4 in Huh7.5.1 cells. Statistical significance was determined by a two-tailed student t-test. Data are presented as mean values ± SD (n = 3 biologically independent experiments; * = p ≤ 0.05, ** = p ≤ 0.01).
