Fig. 3: Syngap1 expression promotes perceptual learning in a whisker-dependent discrimination task.
From: Syngap1 promotes cognitive function through regulation of cortical sensorimotor dynamics
a Schematic of the pole-location discrimination task. b Fraction of total trials correct, False Alarm (FA) rate, Hit rate, and d’ during the acquisition phase of the task. c Schematic of the reduced stimulation phase of the task. Wildtype (wt, n = 9) and Syngap1+/- (+/-, n = 8) d Fraction of total trials correct, False Alarm (FA) rate, Hit rate and d’ during the reduced stimulation phase. Wildtype (wt, n = 9) and Syngap1+/- (+/−, n = 7). Number of animals per genotype is indicated in parentheses. Shaded areas represent the standard error of the mean (S.E.M.). A mixed-effects model and Two-way RM ANOVA were used. P-value for main effects and interaction are indicated as n.s.: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. e–i Whisker dynamics in free air and during object exploration. Superimposition of 40 frames representing whisking in wt mouse acquired at 500 Hz illustrating free whisking of a single whisker, scale bar = 5 mm (e). Representative traces of whisker angle in wt (blue) and Syngap1+/- (red). Quantification of whisker setpoint (g), amplitude (h) and velocity (i) of the protraction (prot.) and retraction (ret.) whisking phase during a 30 second recording window. j–o Active touch dynamics of a single whisker for the first 30 seconds of pole presentation, superimposition of 40 frames representing active touch, scale bar = 5 mm (j). Representative traces of whisker curvature (k) and quantification of area under the curve (AUC) for whisker curvature (l), average touch duration (m) and the total number of peak protractions detected for each individual touch event for wildtype (wt, n = 1144) and Syngap1+/- (+/−, n = 1541) (n) and their respective animal average (o). Error bars represent standard error of the mean (S.E.M.) and number of animals for wildtype (wt, n = 17), and Syngap1+/- (+/−, n = 19) is also indicated in white within the bar graph and/or in parentheses. All statistical tests were 2-sided. P-value are indicated as n.s.: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Detailed statistics are provided in Supplementary Table 3. Source data are provided as a Source Data file.
