Fig. 5: Impaired thalamocortical network dynamics during active sensing in Syngap1^+/- mice.

a Experimental design and timeline during multi-channel silicon probe recordings. b Schematic of the neural circuit and probe insertions (M1: motor cortex, S1: somatosensory cortex and TH: sensory thalamus). c Representative DiI (red) staining of probe implantation in M1, TH, S1, (left to right) and overlayed onto the Allen Brain atlas d Track reconstruction for all wt and Syngap1+/− (+/−). e Average spike rate (spikes/seconds) for extracted cluster including multiunit activity for both wt and Syngap1+/− (+/−) in M1, TH, and S1. (Small dots: units and their distribution (half violin plot), larger dots: animal means, and largest dots: group averages). f Raster and PSTH examples of firing for multi-units from M1 (top row), S1 (middle), TH (bottom) in wt and Syngap1^+/- mice. Shaded areas represent the standard error of the mean (S.E.M.). g–i Subspace analysis of neural communication in Syngap1+/- mice across and within cortical regions. g Schematics of neural subspace dimensionality and predictive performance analysis across neural populations. High-dimensional neural activity in a source population is mapped onto a lower-dimensional subspace that is optimized for predicting the target population activity. h The subspace dimensionality in the source population for predicting neural activity in the target population was determined by selecting the smallest number of dimensions at which the predictive performance, measured by 1-NMSE (Normalized Mean Squared Error), remained within one standard error of the mean (SEM) from the best performance. Bar plots display minimal subspace dimensionality with error bars representing SEM across different randomized choices of the source and target neural populations (see Methods). To evaluate the significance of differences in subspace dimensionality between genotypes a Monte Carlo permutation test was applied. i Predictive performance, measured as 1-NMSE (normalized mean squared error), of the target population activity based on the source population activity across the same-region and cross-region pairs. All statistical tests were 2-sided. P-value are indicated as n.s.: p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.