Fig. 2: Methane-cycling microbes are conserved across wetlands. | Nature Communications

Fig. 2: Methane-cycling microbes are conserved across wetlands.

From: Metabolic interactions underpinning high methane fluxes across terrestrial freshwater wetlands

Fig. 2

a Wetland type is an important control on microbiome membership and structure, despite differences in sampling strategies and geographic locations. 16S rRNA amplicon data (n = 1112 samples) on soil microbial communities from marsh and swamp samples cluster together (rectangles and diamonds, most right side) and are statistically distinct from fen (triangle, middle, and most left side) and bog (circle, middle) microbial communities. b Core methane-cycling members across distinct wetlands calculated from 16S rRNA data. Heatmap shows the relative abundance of each genus within the methanogen (blue) or methanotroph (red) community across wetlands. To illuminate the metabolic features of these core taxa in high methane-emitting wetlands, we utilized the Multi-Omics for Understanding Climate Change (MUCC) v 2.0.0 database, with 140 MAGs assigned to our core taxa. Genome counts per genus are shown in the bar chart (black). MAGs were identified as methanogens if they encoded any genes of the Methyl Coenzyme Reductase (mcrABG) and/or Heterodisulfide reductase (hdrABCDE) complexes and as methanotrophs if they contain a gene that encodes a methane monooxygenase (Supplementary Data S4). Source data are provided as a Source Data file.

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