Fig. 2: Quantification of DH loading on a roadblocked ARS1 origin template using MCM RA mutants.
From: Unidirectional MCM translocation away from ORC drives origin licensing
a DH formation was assessed by negative stain analysis and 2D averaging. Data represent mean ± SD. N = 3 independent experiments. Source data are provided as a Source Data file. b Representative 2D averages and cartoon models corresponding to detected MCM loading intermediates. c Schematic representation of the relative effects of RA mutations on the efficiency of DH formation around the MCM ring view from the C-terminus. The percent of activity retained after mutation is shown next to the targeted ATPase sites, with 0–5% of WT indicated by “--”; 5–15%, “-”; and 15–25%, “+”. OC-MC ORC-Cdc6-MCM-Cdt1 complex, OCCM ORC-Cdc6-Cdt1-MCM complex, MO MCM-ORC complex, MOC-MC MCM-ORC-Cdc6-MCM-Cdt1 complex, DH MCM double hexamer.
