Fig. 6: MCM recruitment and DH formation by MCM h2i and PS1 hairpin mutants.
From: Unidirectional MCM translocation away from ORC drives origin licensing
a, b Silver-stained SDS-PAGE analysis of MCM recruitment (ATPγS, low salt wash) and DH formation (ATP, high salt wash) using streptavidin-coated magnetic beads and a roadblocked ARS1 origin template for MCM h2i (a) and PS1 (b) hairpin mutants. This experiment was performed once. c Negative stain analysis and 2D averaging of DH loading reactions in solution, with bar graphs showing mean ± SD. N = 3 independent experiments. Source data for all panels are provided as a Source Data file. OC-MC ORC-Cdc6-MCM-Cdt1 complex, OCCM ORC-Cdc6-Cdt1-MCM complex, MOC-MC MCM-ORC-Cdc6-MCM-Cdt1 complex, DH MCM double hexamer.
