Fig. 3: BEN1 impacts cellulose synthesis.
a Five-day-old seedlings grown on 1/2 MS media were transferred to soil and grown for 18 days in the greenhouse. Scale bar = 2 cm. b Rosette leaves were harvested from plants as those in (a) and cellulose levels were measured. Values are mean ± SD. Significance was determined by one-way ANOVA followed by a Tukey’s test (p < 0.05, n = 3). c Three-day-old seedlings grown on 1/2 MS media were transferred to media with 2 nM isoxaben for an additional 4 days. Scale bar = 0.5 cm (root elongation), scale bar = 0.5 mm (root tip area). d Root elongation after transfer to plates as indicated. Values are mean +/− SD. Significance was determined by one-way ANOVA followed by a Tukey’s test (p < 0.05, n ≥ 15). e Venus-CC1 and YFP-CESA6 in ben1 or wild-type were imaged following BFA (50 μM for 30 min) or DMSO (control) treatments. Scale bar = 20 µm. f BFA body distribution across cells in the root area, percentage area (% area; upper panel) and count per mm2 (lower panel) were used for statistical analyses. Values are mean±SD. Significance was determined by a two-sided t-test (* p < 0.05, ** p < 0.01, images from independent seedlings n > 5). g Venus-CC1 in ben1 or wild-type backgrounds were imaged following CHX + ES9-17/DMSO + BFA treatment (CHX 50 µM for 60 min, followed by ES9-17 30 µM /DMSO for 30 min, then BFA 50 µM for 30 min.). Scale bar = 20 µm. h BFA body distribution was reflected by the number of BFA bodies per cell; ≥100 cells were counted from images of independent seedlings n > 5 for each experiment repeated four times. Data from each replicate is shown in a different color. Significance was determined by the Kruskal-Wallis test, followed by pairwise comparison using Wilcoxon test, *** p < 0.001.
