Fig. 2: Dynamic immune recognition of NP corona complexes. | Nature Communications

Fig. 2: Dynamic immune recognition of NP corona complexes.

From: Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles

Fig. 2

a Flow cytometry mean fluorescence intensity (MFI) quantification of macrophage surface markers CD86, CD80, and PD-L1 after treatment with pristine PLGA NPs or NP coronas for 30-min or 24-h (n = 3 biological replicates per timepoint). Representative histograms of 24-hour timepoints are shown below. Data is presented as mean values +/- SD. Statistical significance was determined with a two-way ANOVA using a Dunnett’s test with No Treatment set as control. b TNFα secretions over time from macrophages after PLGA NP or NP corona treatment (n = 3 biological replicates per timepoint). Data is presented as mean values +/- SD. c Multiplex cytokine analysis in the supernatants of NP corona-treated macrophages after 3-hour incubation (n = 3 biological replicates). Data is presented as mean values +/- SD. Statistical significance was determined with a two-way ANOVA using Tukey post hoc test. d Spearman correlation matrix of the macrophage multiplex cytokine analysis. Colors range from dark red, indicating a strong positive correlation between sample cytokines, to dark blue, indicating strong negative correlation. e Organ biodistribution of Cy5.5-labeled PLGA NPs coated in various plasma coronas 3-h post i.v. injection in naïve C57BL/6 mice. Heart, liver, lung, spleen, kidney, and brain were isolated and analyzed via in vivo imaging system (IVIS) for NP fluorescence. Representative organ samples are presented from saline (S), PLGA (P), NaïvePlas_PLGA (N), 3hrPlas_PLGA (3), and 8hrPlas_PLGA (8) treated mice. Percent of total radiant efficiency is presented in the graph below (n = 2 mice per group). No fluorescence was detected in brain samples. f TNFα plasma concentration from NP corona-treated IVIS mice (n = 2 mice per group, 3 technical replicates each). Data is presented as mean +/- SD. Significance was calculated using a two-way ANOVA with Tukey post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001. ns, not significant (P > 0.05). Source data are provided as a Source Data file.

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