Fig. 4: 3hrPlas coronas signal pro-inflammatory cytokine secretions through TLR4 activation of NF-ĸB. | Nature Communications

Fig. 4: 3hrPlas coronas signal pro-inflammatory cytokine secretions through TLR4 activation of NF-ĸB.

From: Inflammatory disease progression shapes nanoparticle biomolecular corona-mediated immune activation profiles

Fig. 4

a. NF-kB activation in RAW-Blue reporter cell line upon incubation with PLGA NP coronas. NF-ĸB activation was normalized to the LPS positive control (n = 3 biologically independent experiments). b TNFɑ secretions from macrophages treated with PLGA NP coronas in the presence or absence of Bay11–7082, an NF-ĸB inhibitor at 10 µM (n = 3 biological replicates), p < 0.0001. c. TNFɑ secretions from macrophages treated with PLGA NP coronas in the presence or absence of TJ-m2010-5, a MyD88 inhibitor at 30 µM (n = 3 biological replicates). d. TNFɑ secretions from macrophages (n = 3 biological replicates) compared to TLR4 knock-out (K/O) macrophages (n = 6 biological replicates) treated with PLGA NP coronas. e. Schematic illustration of the proposed corona-dependent pro-inflammatory cytokine signaling. Created in BioRender. Shaw, J. (2025) https://BioRender.com/v63l645. Data sets are presented as mean ± S.D. Significance for all graphs were calculated using a one-way ANOVA with Tukey’s post-hoc test. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001. ns, not significant (P > 0.05). Source data are provided as a Source Data file.

Back to article page