Fig. 4: Transcriptome-wide analysis of synEPOR-edited cells.
From: Engineering synthetic signaling receptors to enable erythropoietin-free erythropoiesis
A Schematic of well-characterized endogenous EPO + EPOR signaling effects vs. undefined BB+synEPOR signaling effects. Created in BioRender. Lesch, B. (2025) https://BioRender.com/z58z349. B Transcripts per million (TPM) from RNA-Seq with annotations for globin, EPOR, and synEPOR genes. C Volcano plot comparing unedited and edited HSPCs at d14 of differentiation v. unedited HSPCs at d0. Dashed lines are drawn at +/−1 log2 fold change and adjusted p-value = 0.01 by Wald test. Total number of significantly down- and upregulated genes is shown in top left and top right of each plot, respectively. D Volcano plot comparing edited HSPCs at d14 +BB v. unedited HSPCs at d14 +EPO. Dashed lines are drawn at +/−1 log2 fold change and adjusted p-value = 0.01 by Wald test. Total number of significantly down- and upregulated genes is shown in top left and top right of each plot, respectively. E Principal component analysis of all conditions with covariance ellipses. F Summary of gene ontology (GO) enrichment analysis comparing all d14 conditions v. d0 control. Top 50 differentially expressed genes were used as input. Plotted are significantly enriched GO pathways (Benjamini-Hochberg False Discovery Rate adjusted p-value ≤ 0.05) that were binned into broader categories with enrichment score derived by Enrichr software. Count refers to the number of genes within each GO pathway that contributed to enrichment. Source data are provided as a Source Data file.
