Fig. 7: Confirmation of covalent bond formation during bacterial copolymerization of glucose and glycosylate dyes.

a Schematic representation of the confirmation process with the help of cellulase-catalyzed biodegradation (S3-BC as an example). b Fluorescence spectrum (left) and representative mass analysis (right) of hydrolysate for S2-BC. HRMS in the positive ion reflective mode demonstrated the [M + K]⁺ peak of S2-hybrid cellobiose (m = 1, n = 2) and [M + H]⁺ peak of S2-hybrid cellotriose (m = 1, n = 3) at a mass-to-charge ratio (m/z) of 892.6753 and 1016.8156, respectively. c Fluorescence spectrum (left) and representative mass analysis (right) of hydrolysate for S3-BC. HRMS in the positive ion reflective mode demonstrated the [M + K]⁺ peak of S3-hybrid cellobiose (m = 1, n = 2) and [M + Na]⁺ peak of S3-hybrid cellopentaose (m = 1, n = 5) at a mass-to-charge ratio (m/z) of 996.3273 and 1466.4434, respectively. HRMS, high-resolution mass spectrometry. Source data are provided as a Source Data file.