Fig. 1: Urolithin C metabolism is limited to a subset of Enterocloster spp.
From: Diet-derived urolithin A is produced by a dehydroxylase encoded by human gut Enterocloster species
a Reaction scheme of uroC dehydroxylation by gut resident Enterocloster spp. via unknown enzymes. b LC-MS screen of Enterocloster spp. type strains for uroC dehydroxylation activity. UroC (100 μM) was added to cultures (in mABB + H media) at the start of growth, and urolithins were extracted after a 24 h anaerobic incubation and then analyzed by LC-MS. Representative chromatograms (λ = 305 nm) for each experimental group (left, from one representative biological replicate). The same scale was used for each chromatogram. Quantification of urolithin peak areas relative to a salicylic acid internal standard (IS) (right, n = 3 biological replicates). Coloring for uroC-metabolizing is consistent throughout the manuscript: E. asparagiformis (red), E. bolteae (blue), E. citroniae (orange). Data are represented as mean ± SEM. Source data are provided as a Source data file.
