Fig. 3: Urolithin C metabolism correlates with ucd operon prevalence in gut bacteria.
From: Diet-derived urolithin A is produced by a dehydroxylase encoded by human gut Enterocloster species
a Maximum likelihood phylogenetic tree (left) of Enterocloster spp. and catechol-metabolizing Eggerthellaceae based on the Genome Taxonomy Database (GTDB). Bacteria that convert uroC to uroA are labeled with gray squares. NBCI Multiple Sequence Aligner viewer hits for BLASTn searches using the E. bolteae DSM 15670 ucd operon nucleotide sequence as a query against the NCBI refseq_genomes database (right). Domain annotations for each gene are denoted below according to InterPro annotations for corresponding proteins. ND, Not detected; Moco, Molybdenum cofactor. b, c In vitro metabolism of uroC by Enterocloster pacaense (Ep). UroC (100 μM) was added to cultures (in mABB + H media) at the start of growth, and urolithins were extracted after 24 h anaerobic incubation and then analyzed by LC-MS. b Representative chromatograms (λ = 305 nm) for each experimental group (from one representative biological replicate). The same scale was used for each chromatogram. c Quantification of urolithin peak areas relative to a salicylic acid internal standard (IS) (n = 3 biological replicates). Data are represented as mean ± SEM. Source data are provided as a Source data file.
