Fig. 5: The catechol moiety of uroC delays Enterocloster spp. growth.
From: Diet-derived urolithin A is produced by a dehydroxylase encoded by human gut Enterocloster species
a Growth curves (optical density (OD) at 620 nm) of uroC-metabolizing Enterocloster spp. treated with increasing concentrations of uroC in rich mABB + H media (7.7 μM hemin) (n = 3 biological replicates). Data are represented as mean ± SEM. b Quantification of the difference in lag time compared to the DMSO control for growth curves in (a). Data are represented as mean ± SEM; lines were fitted using simple linear regression. c Quantification of the difference in lag time of Enterocloster spp. grown in mABB (no added iron) or mABB + H (7.7 μM hemin) compared to respective DMSO controls for growth curves in Supplementary Fig. 14a, b (n = 3 biological replicates). Data are represented as mean ± SEM; repeated measures two-way ANOVA (matching by biological replicate) with Tukey’s multiple comparisons test. Statistical significance is denoted in the plot with exact P-values when 0.05 > P ≥ 0.0001 (****). d Structures of tested urolithins. e Quantification of the difference in lag time of Enterocloster spp. grown in mABB (no added iron) treated with 100 μM urolithins in (d) compared to respective DMSO controls (n = 3 biological replicates). Data are represented as mean ± SEM; repeated measures two-way ANOVA (matching by biological replicate) with Tukey’s multiple comparisons test (main treatment effect). P-values for comparisons are provided in the Source data file. f Growth curves (OD at 620 nm) of uroC-metabolizing Enterocloster spp. grown in mABB media and treated with DMSO (vehicle), 100 μM of uroC, uroA, or 8,9-di-O-methyl-uroC (n = 3 biological replicates). Data are represented as mean ± SEM. g Quantification of the difference in lag time compared to the DMSO control for growth curves in (f). Data are represented as mean ± SEM; repeated measures two-way ANOVA (matching by biological replicate) with Tukey’s multiple comparisons test. Statistical significance is denoted in the plot with exact P-values when 0.05 > P ≥ 0.0001 (****). Source data and statistical details are provided as a Source data file.
