Fig. 4: Distinct in vivo bone marrow homing of Allo15CAR33-NKT cells mediated by CXCR4/CCR5 expression.
a Experimental design to study the in vivo PK/PD of Allo15CAR33-NKT cells in a xenograft NSG mouse model. The therapeutic cells were labeled with FG. Created in BioRender. LI, Y. (2025) https://BioRender.com/u95q769. b BLI images showing the presence of therapeutic cells in experimental mice over time. Ventral and left-side views are shown. c Quantification of (b) (n = 3 from three experimental mice). TBL, total body luminescence; p/s, photons per second. d BLI images showing the biodistribution of Allo15CAR33-NKT and CAR33-T cells in representative experimental mice. Ventral and left-side views are shown. e Quantification of therapeutic cell tissue (i.e., bone marrow and other tissues) distribution (n = 3 from three experimental mice). f FACS detection of therapeutic cells in the bone marrow of experimental mice 30 days after cell injection. g Quantification of (f) (n = 5 from five experimental mice). h FACS measurement of surface CXCR4 and CCR5 expressions in the indicated therapeutic cells. i Quantification of (h) (n = 5 from five experimental mice). j Schematic illustrating the distinct in vivo bone marrow homing capacity of Allo15CAR33-NKT cells mediated by CXCR4/CCR5 expression. Created in BioRender. LI, Y. (2025) https://BioRender.com/g04r306. Representative of 2 experiments. Data are presented as the mean ± SEM. ***p < 0.001; ****p < 0.0001, by two-tailed Student’s t test (g, i). Source data and exact p values are provided as a Source Data file.
