Fig. 7: Association of TBK1-FBXO3-TMEM192-TAX1BP1 axis with LLOMe-induced lysophagy in Drosophila model.

a Cotreatment of Baf, BC-1215, or GSK8612 with LLOMe showed decrease of mCherry⁺/SEpHluorin^- Gal3 puncta in larva fat body. The number of mCherry/SEpHluorin puncta per cell was quantified. Data are presented as the mean ± SEM (n = 150, *p = 0.0003, **p < 0.0001). A total of 150 individual larva fat body cells were counted. Scale bar: 20 µm. b Feeding third-instar larvae of control (Dcg-Gal4/+) and Slmb knockdown (Dcg>Slmb Ri) flies were fed with normal fly food containing LLOMe. After a 2-h feeding period, larvae in the wandering stage were dissected, costained with anti-ubiquitin and anti-LAMP1 antibodies, and subsequently imaged using confocal microscopy. The colocalization of LAMP1 and ubiquitin staining was analyzed by Pearson’s correlation coefficient and line colocalization. The fluorescence intensities of both LAMP1 and Ubiquitin were quantified. Data are presented as the mean ± SEM, with each dot in the plot representing an individual fat body cell (n = 30, *p < 0.0001). Scale bar: 20 µm. All experiments were replicated three times. Two-tailed unpaired t-test were used to analyzed all the data. Source data are provided as a Source Data file.