Fig. 3: Impact of hemocyte depletion on microbiota. | Nature Communications

Fig. 3: Impact of hemocyte depletion on microbiota.

From: Anopheles gambiae phagocytic hemocytes promote Plasmodium falciparum infection by regulating midgut epithelial integrity

Fig. 3

a The midgut microbiota was analyzed in NT, LPSM-injected, and CLD-injected mosquitoes fed on sucrose or at 24 and 48 h post-blood feeding. b Bacterial load in the hemolymph of NT, LPSM-injected, and CLD-injected mosquitoes was measured at 24 and 48 h after blood feeding. c Model showing that hemocytes restrict the midgut microbiota load and control systemic infections produced by midgut microbiota leakage. Each experiment had three biological replicates; data were pooled to generate the graph. Data are represented as medians with maximum and minimum value. a Sucrose (n = NT, 32; LPSMs, 32; CLDS, 31), Blood (24 h) (NT, LPSMs, and CLDs, n = 36), Blood (48 h) (NT, LPSMs, and CLDs n = 32); b Blood (24 h) (n = NT, 20; LPSMs, 22; CLDs, 24), Blood (48 h) (n = NT, 17; LPSMs, 21; CLDs, 21). Statistical analysis was performed using the Kruskal–Wallis test with the two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli. Statistical differences: *p < 0.05; nd not discovery. Source data and detailed statistical analysis are provided as a Source Data file. Schematic illustrations of the experimental strategy in Fig. (3a, b), and the model of Fig. 3c were created by Victor Cardoso-Jaime using Microsoft PowerPoint Version 16.92.

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