Fig. 6: In silico chromosome ancestry painting of a triploid Cavendish cultivar genome assembly and comparison with ancestral contributor assemblies.
From: Unravelling genomic drivers of speciation in Musa through genome assemblies of wild banana ancestors
Circular representation of the Cavendish haplotype assembly (Baxijiao accession29) with: a the distribution of tandem repeats and SIRE/Maximus transposable elements along chromosomes (color code from Fig. 5), b In silico chromosome painting according to the ancestral contributors (color code from Fig. 2), c In silico chromosome painting according to suggested parental gametes37 with black for the 1x gamete and orange and brown for the two haplotypes of the 2x gamete. d Comparison of the assembled Cavendish chromosomes that should have the 1/4, 1/7 and 3/8 rearrangements on the basis of their ancestral origin, with the reference rearranged structures found in ancestral contributor assemblies. The reference structure used for the 1/4 reciprocal translocation corresponds to M. a. ssp. malaccensis24 (M. a. malaccensis h1 chr1T4 and chr4T1), that used for the 1/7 reciprocal translocation corresponds to Pisang Madu H2 (P. Madu H2 chr1T7 and chr7T1) and that used for the 3/8 reciprocal translocation corresponds to M. a. ssp. zebrina (M. a. zebrina chr3T8 and chr8T3). Dashed lines indicate chromosomes from the Cavendish assembly that were compared. Translocated segments on the reference translocated structure are indicated by vertical black bars. Gray shapes locate the syntenic regions. Stars indicate the translocation breakpoints that are present in the assembly. Green, red and blue dots on d locate the BACs used for BAC-FISH analysis on metaphase chromosomes of Cavendish (Grande Naine accession) to validate the presence of the 1/4 and 1/7 e and 3/8 f translocated structures in Cavendish. White horizontal bars in e and f represent 5.1 µm. White dashed line separated chromosomes from another cell. Source data are provided as a Source Data file.
