Fig. 3: Time course and donor-to-donor variability of insulin-stimulated phosphorylation for selected insulin targets in human myotubes.

a Schematic insulin signaling of established insulin-regulated kinases, substrates, and main regulatory phosphorylation sites. Quantitated phosphosites for AKT are labeled in red, and identified sites without quantification shown in gray. b Phosphorylation kinetics based on quantification of phosphopeptides for 28 selected targets. Abundance values for individual donors (symbols) and mean values (red lines) are shown. The individual abundance values were used to calculate the average Pearson’s correlation coefficient r as a measure of variability between donors. Targets with low variability (r < 0.8) are indicated by blue color in (a). c Representative Western blot analysis (n = 3 donors) of selected insulin-regulated phosphosites. Source data are provided as a Source Data file.