Fig. 5: Inhibitory synaptic caspase-3 activation leads to complement tagging.
From: Nonapoptotic caspase-3 guides C1q-dependent synaptic phagocytosis by microglia
a, c Immunohistochemical images of C1q and C3 in the middle molecular layer 6 hours after febrile seizures (FSs). b, d Density of C1q and C3 puncta in the middle molecular layer 6 hours after FSs. Mean ± SD, n = 5 mice, two-sided unpaired t test. e–g Images displaying colocalized inhibitory presynapses with PSVue (PS) and cleaved caspase-3 or C1q or C3 6 hours after FSs (arrows). DEVD refers to the caspase-3 inhibitor Z-DEVD-FMK. h–j Density of inhibitory presynapses colocalized with PSVue and cleaved caspase-3 or C1q or C3 6 hours after FSs. Mean ± SD, n = 5 mice, Tukey’s test after one-way ANOVA. Source data are provided as a Source Data file.
