Fig. 6: The 40S ribosomal subunit recycling complex is associated with ERMCS.

a Immunostaining of mitochondrial markers showing the regulation of ERMCS genes in muscle tissue of Usp10 and rin OE flies. Control (w-) serves as a negative control in each genetic background. Mitochondrial morphology is visualized by mitoGFP in Usp10 OE, and by ATP5α signal in rin OE samples. b Quantification of mitochondrial size shown in (a). Mitochondria counts were obtained from three samples (n = 3, 7 areas counted per sample). c Immunostaining of mitoDsRed (a mitochondrial-targeted DeRed reporter) and KDEL-eGFP (An eGFP and KDEL fusion protein, ER marker) showing the regulation of mitochondria-ER gathering by RQC genes in fly muscle tissue. Control (w-) serves as a negative control. d Quantification of the number of gathering sites per mitochondrion in each genotype shown in (c). Gathering site counts were obtained from three samples (n = 3, 7 areas counted per sample). One-way ANOVA test (two-sided) followed by post hoc Dunnett’s multiple comparisons test 95% confidence interval (CI) was used in (b, d, e, g, i, k). Immunostaining of mitoDsRed, KDEL, and components of the 40S ribosomal subunit recycling complex, Usp10 (e), rin (g), dZnf598 (i), and Fmr1 (k), showing their colocalizations with ERMCSs in fly muscle tissue. Mitochondrial morphology is visualized by mitoDsRed and ER morphology is visualized by KDEL-eGFP. White dashed lines indicate the regions where colocalization analysis was performed in f, h, j, k, respectively. Three samples (n = 3) were analyzed. f, h, j, l Colocalization analyses of images shown in e, g, i, k, respectively. Black arrows indicate the colocalization of Usp10 (e), rin (g), dZnf598 (i) and Fmr1 (k) with ER signals close to mitochondria. All scale bars, 5 µm. Data are means ± SEM. The p values are included in the figure. Source data are provided as a Source Data file.