Fig. 4: Structures of surface-stabilized protein condensates with interfacial protein cages.
From: Size-controlled assembly of phase separated protein condensates with interfacial protein cages
a Confocal Z-stack 3D representation of mi3-LAF condensates (LAF: magenta, mi3: green). b Negative-stained TEM images of mi3-LAF (left) and mi3-PSH (right) condensates. c Cryo-EM images of mi3-LAF (left) and mi3-PSH (right) condensates. d Reconstructed tomograms of mi3-LAF condensates. Four images of equal Z-distances (~40 nm) are shown below to demonstrate cage distributions at the bottom/middle/top of the condensate. Thick black dots are 10 nm gold nanoparticles used as a fiducial marker. e 3D volume models of mi3-LAF condensates (left: top view of the whole tomogram, right: front and side view of selected condensates). mi3 cages are shown as gray spheres. f Tomogram slices of mi3-PSH condensate with ferritin cages (red arrows) located inside the condensate. g Contact angles of mi3 cages on LAF condensates with a representative tomogram slice image. Exemplary cages and contact condensate surfaces are indicated with red dotted circles and lines, respectively. h Side views of 3D volume models of mi3-stabilized LAF (top) and PSH (bottom) condensates. Scale bars, a 5 μm. b, c, d, e, f 100 nm. g 25 nm. h 50 nm. Source data are provided as a Source Data file.
