Fig. 5: Dynamic behaviors of cage-stabilized protein condensates.
From: Size-controlled assembly of phase separated protein condensates with interfacial protein cages
a Confocal images of separately labeled (Cy5 magenta, Cy3 cyan) cage-stabilized condensate mixtures after 10 min incubation upon mixing (top: PS-RL48 condensates, bottom: PSH condensates). b Confocal images of cage-stabilized condensates with externally added client biomolecules (DNA (yellow) and mCherry-6His (red)). c Confocal images of HF oligo-PSH condensates (with or without protein G fusion to PSH) assembled with antibody (cyan). d Sucrose gradient centrifugation of cage-stabilized PSH condensates. A picture of sucrose gradient centrifuged HF oligo-PSH condensates with different sizes with schematics and exemplary confocal images of isolated PSH condensates in sucrose solution are shown. e Fluidity change of cage-stabilized condensates. DNA recruited into fluidic PS-RL48 condensates induces additional PS-RL48 clustering around DNA, resulting in decreased size and fluidity of condensates (left: schematics). FRAP recovery profiles (red: PS-RL48, blue: recruited DNA, n = 10 condensates) and confocal images (PS-RL48) of the bleached DNA-containing PS-RL48 condensates are shown in the middle. A FRAP profile for fluidic PS-RL48 condensates is shown as a control (black). Confocal images for separately labeled, DNA-recruited PS-RL48 condensate mixtures after 60 min incubation (right top) and 4X diluted condensates (right bottom) are shown in the right. Data are presented as mean values ± 0.5 s.d. f Confocal images of HeLa cells treated with HF oligo-PSH, mi3-PSH, and mi3-LAF condensates. Scaffold (magenta), cage (green), and DAPI (blue). Scale bars, a–e 5 μm. f 10 μm. Source data are provided as a Source Data file.
