Fig. 9: Lactate directly modulates DC metabolism and dampens DC function. | Nature Communications

Fig. 9: Lactate directly modulates DC metabolism and dampens DC function.

From: MCT1 lactate transporter blockade re-invigorates anti-tumor immunity through metabolic rewiring of dendritic cells in melanoma

Fig. 9

PanDCs purified from HD blood were co-cultured with rising concentrations of sodium L-lactate (10, 25 and 50 mM) for 45 min and stimulated or not with a mixture of TLR-L for 4 h. DC subset metabolism was then studied by flow cytometry using the SCENITH method, and their cytokine/chemokine production was measured in supernatants using Luminex. A Comparative levels of global metabolism (MFI of puromycin) for each DC subset (unstimulated, n = 6 donors). B Glycolytic or mitochondrial dependencies and capacities of DC subsets (unstimulated, n = 6 donors). C, D Cytokine/chemokine production in culture supernatants (n = 6 per group). For box plots (A, B), the central line indicates median, the box contains the 25th to 75th percentile, and whiskers the min and max values. P-values were calculated using a one-way Friedman test followed by Dunn’s multiple comparison post-tests (A, C, D), or non-parametric two-way ANOVA or a mixed effect model followed by Tukey’s multiple comparison post-tests (B). Only significant statistics are displayed on graphs. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Source data are provided as a Source Data file.

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