Fig. 3: Membrane bending induced by RCD-1.
From: Mechanisms of RCD-1 pore formation and membrane bending
a Bent membranes of DOPC: DOPS = 6: 4 or DOPC: DOPS = 8: 2 ranging from small, sphere-like structures (i) to large, irregular, and flat forms (ii) (i-ii, experimental repeats, n = 10). (iii) High-resolution AFM image of the bent membrane of DOPC: DOPE: DOPG = 5: 3: 2, with small particles on the surface (A, B, C, D) (experimental repeats, n = 3). The particles occur at heights of 3.0 nm (n = 24) (iv) and are 5.6 ± 0.5 nm (mean ± SD, n = 24) in width (v). The right panels show the height profile along the dashed line. b Confocal fluorescence microscope images of GUV local membrane bending upon addition of RCD-1. The GUV composition is DOPC: DOPS: Liss rhod PE(18:1) = 8: 2: 0.005. Green represents FITC (dextran) in the solution and red represents 18:1 Liss rhod PE of GUV (experimental repeats, n = 3).
