Fig. 5: Time-lapse AFM reveals a two-step membrane bending process at low concentration of RCD-1.
From: Mechanisms of RCD-1 pore formation and membrane bending
a Membrane bending underwent curvature expansion at first, followed by an increase in curvature accompanied by a reduction in the size of bent area (experimental repeats, n = 5). Specifically, 250 nM RCD-1 was co-incubated with DOPC: DOPS = 8: 2 to form some initial bending islands, followed by a switch to 50 nM RCD-1 at 10 min. Yellow arrows and circles represent the same position, while green and blue arrows represent new formed rings. b, c The height profiles of the indicated membrane bending sites.
