Fig. 6: Membrane stiffness measurement by AFM force spectroscopy and micropipette aspiration.
From: Mechanisms of RCD-1 pore formation and membrane bending
AFM topographs of RCD-1 incubated with various lipid membranes of DOPC: DOPS = 8: 2 (a) (a, experimental repeats, n = 10), DOPC: DOPE: DOPS = 6: 2: 2 (b), DOPC: DOPE: DOPS = 5: 3: 2 (c), PC(22:1): DOPS = 8: 2 (d), DOPC: DOPE: DOPA = 6: 2: 2 (e), DOPC: DOPE: PA(18:0) = 6: 2: 2 (f). b–f Experimental repeats, n = 3. g Illustration of how force spectroscopy was used to measure membrane rigidity. h Bending moduli of the six lipid compositions, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a-f represent the lipid components of (a–f). The AFM force spectroscopy experiments included n = 73, 51, 55, 62, 59, and 56 force curves for a-f, respectively. For micropipette aspiration experiments, n = 10, 10, 9, 9, 9, and 10 GUVs for a-f, respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.
