Fig. 7: RCD-1 oligomerization in fungal membranes.
From: Mechanisms of RCD-1 pore formation and membrane bending
AFM topographies of RCD-1 incubated with various different fungal membranes: model fungal cell membrane-DOPC: DOPE: DOPA: DOPS: CL(18:1) = 30: 28: 20: 4: 18 (a) (weight ratio); model fungal mitochondrial membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 37: 43: 1: 8: 4: 6: 1(b); model fungal mitochondrial outer membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 40: 36: 4: 9: 5: 5: 1(c); model fungal mitochondrial inner membrane-DOPC: DOPE: DOPA: DOPI: DOPS: CL(18:1): DOPG = 32: 30: 1: 5: 5: 25: 2(d); model modified fungal cell membrane- DOPC: DOPE: PA(18:0): DOPS: CL(18:1) = 30: 28: 20: 4: 18(e); model modified fungal mitochondrial inner membrane- DOPC: DOPE: DOPA: DOPI: DOPS: Heart CL(18:2): DOPG = 32: 30: 1: 5: 5: 25: 2(f) (a–f, experimental repeats, n = 3). Membrane bending occurred in the model fungal cell membrane (a) and model fungal mitochondrial inner membrane (d). Change the PA from 18:1 to 18:0 for artificial fungal cell membrane, bending disappeared in model modified fungal cell membrane (e). Change the CL from 18:1 to 18:2 (Heart CL), more bending occurred in the model modified fungal mitochondrial inner membrane (f). g Bending moduli of the fungal membranes, measured by AFM force spectroscopy (black) and micropipette aspiration (red). Symbols a-f represent the lipid components of (a–f), while symbol g represents the yeast extract polar lipid. The AFM force spectroscopy experiments included n = 37, 38, 44, 41, 47, 31, and 37 force curves for a-g, respectively. For micropipette aspiration experiments, n = 9, 9, 10, 10, 9, 10, and 9 GUVs for a-g, respectively. In box plot visualizations, the central line represents the median, while the bottom and top edges of the box denote the 25th and 75th percentiles, respectively.
