Fig. 3: 3D-DAT of glassfrog and mouse in vivo.

a Multispectral images of glassfrog (Hyalinobatrachium fleischmanni) by traditional PA imaging and DPAT, acquired at 532 nm (in green), 673 nm (in red), and 1064 nm (in blue). Ab. V, abdominal vein; Br. V, brachial vessels; E, eyes; L.sac, lymphatic sac (pelvic and ventral); Tf, Tibio-fibula. The absorption distribution at 673 nm is responsible for the cyan-green coloration in the lymph and lungs of the glassfrogs. b FWHM of the blood vessel marked with the dashed blue line in (a) by TD PA (top) and DPAT (bottom). c SNR of three regions of interest marked as ‘R1’, ‘R2’, and ‘R3’ in (a), using the same background region marked as ‘B’. d Images of the glassfrog by traditional US imaging and DUST. H, heart; L, lungs. e FWHM of the bone marked with the dashed blue line in (d) by TD US imaging (top) and DUST (bottom). f SNR of three regions of interest marked as ‘R1’, ‘R2’, and ‘R3’ in (d), using the same background region marked as ‘B’. g Multispectral imaging of a mouse abdominal region by traditional PA imaging and DPAT, projected along the lateral/elevational view (top) and the elevational/axial view (bottom). DV, deep vessels; SV, superficial vessels. Compared with the traditional PA imaging, DPAT shows a comparable imaging depth and improved elevational resolution. h Corresponding images of the mouse by traditional US imaging and DUST, showing comparable imaging depth but improved elevational resolution by DUST. LB, leg bone; VB, vertebrae. Scale bars, 2 mm.