Fig. 3: Down-regulation of macrophage Pellino1 improves prognosis and suppresses the development of CAC. | Nature Communications

Fig. 3: Down-regulation of macrophage Pellino1 improves prognosis and suppresses the development of CAC.

From: The ubiquitin ligase Pellino1 targets STAT3 to regulate macrophage-mediated inflammation and tumor development

Fig. 3

a Experimental schematics for the AOM/DSS-induced CAC model. b Overall survival curves of WT and Pellino1-mKO male mice in the AOM/DSS model (WT, n = 62; Pellino-mKO, n = 59). c Body weights of AOM/DSS-induced CAC mice were monitored (WT, n = 9; Pellino-mKO, n = 11). *p < 0.05. Exact p values are as follows: Day 40 = 0.0464, Day 43 = 0.034, Day 66 = 0.0189, Day 68 = 0.017, Day 71 = 0.0338, Day 73 = 0.0328, and Day 80 = 0.0284. d (Left) Colon lengths of WT and Pellino1-mKO male mice after AOM/DSS treatment (n = 17). (Right) Images of colon tissues from WT and Pellino1-mKO male mice after AOM/DSS treatment. e Representative images of colon tumor formation in WT and Pellino1-mKO male mice. These images indicate the medial and distal regions of the colon. Scale bar = 1 cm. f (Left) Total number of tumor nodules in the entire colon (n = 12). (Right) Numbers of small tumors (<2 mm) and large tumors (>2 mm) in the entire colon (n = 12). g H&E-stained images of colon sections from WT and Pellino1-mKO male mice. Scale bar = 400 μm. h (Left) Representative immunohistochemical stained images of Ki67, F4/80, and Ly6G in colon tissues of WT and Pellino1-mKO male mice after AOM/DSS treatment. Scale bar = 60 μm. (Right) Numbers of Ki67 (n = 6), F4/80 (n = 5), and Ly6G (n = 4) positive cells. Quantification of cells in the field of view area. i, j Levels of TNFA (WT, n = 5; Pellino-mKO, n = 6), IL6 (n  =  6), IL1B (n  =  6), and IL10 (n  =  4) mRNA were measured in colon tissues of WT and Pellino1-mKO male mice after AOM/DSS treatment using qRT-PCR and normalized to GAPDH expression. k The mRNA expression level of 16s rRNA in spleens of WT and Pellino1-mKO male mice after AOM/DSS treatment. The mRNA expression was assessed by qRT-PCR and normalized to GAPDH expression (n  =  6). Data were represented as mean ± SD in (c, d, f, h, i, j, and k). All statistical comparisons were made using a two-tailed Student’s t-test. Source data are provided as a Source Data file.

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