Fig. 2: IFI16 is a critical gene in regulating cellular senescence-like phenotype in GBM. | Nature Communications

Fig. 2: IFI16 is a critical gene in regulating cellular senescence-like phenotype in GBM.

From: Cellular senescence-associated gene IFI16 promotes HMOX1-dependent evasion of ferroptosis and radioresistance in glioblastoma

Fig. 2

a The GO analysis of the intersection of the different peaks and DEGs between ATAC-seq and RNA-seq of U251R/U251 cells. The p-values were calculated using the hypergeometric test by ClusterProfiler. b The ATAC-seq peaks at IFI16 in U251R/U251. c Western blot assay of IFI16 proteins in GBM cells. d The chord plot of the top five GO terms of the DEGs between U251 and U251R cells. e The GSEA analysis of the regulation of immune system process pathway in RNA-seq results of U251R cells with IFI16 knockdown (shIFI16) and negative control (shNC). f The β-galactosidase assay of U251R and Ln229R cells with shIFI16 (n = 3). An unpaired two-tailed t-test was applied. Data are presented as mean ± SD. g Western blot assay of IFI16, HP1, p21, H3K27ac, and p16 proteins in U251R and Ln229R cells with shIFI16. h Immunofluorescence analysis of Ki67 expression and Ki67-positive area across U251R and Ln229R cells with shIFI16 (n = 3). Scale bars, 20 μm. An unpaired two-tailed t-test was applied. Data are presented as mean ± SD. i RT-qPCR analysis of SASP factors in U251R and Ln229R cells with shIFI16 (n = 3). Data are presented as mean ± SD. j Concentrations of cytokines CCL5, IL-6, MMP-9, and IFN-β in the supernatants of U251R and Ln229R cells with shIFI16 (n = 3). An unpaired two-tailed t-test was applied. Data are presented as mean ± SD. “n” means the number of individual experiments.

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