Fig. 6: ACT with piezoMϕ for P. aeruginosa-induced lung infection.

a Experimental procedures for the antimicrobial efficacy study in a P. aeruginosa-induced lung infection model. A single injection of PBS, Mϕ, piezoMϕ, or activated piezoMϕ was given intravenously 4 h after the infection. b Survival analysis of the mice (n = 6 mice). Survival of mice was determined using Kaplan–Meier and compared using the log-rank test. c Bacteria burden in mice lung with P. aeruginosa infection (n = 3 mice). The infected lungs were collected 24 and 168 h after the injection. d Levels of IL-6 in BALF (n = 3 mice). e Levels of TNF in BALF (n = 3 mice). f Lung histology images of different treatments (PBS, Mϕ, piezoMϕ, or activated piezoMϕ). g Biodistribution of DIR-labeled Mϕ in mice by In Vivo Imaging System (IVIS). Images were recorded at different time points and in main organs (H: heart, L: liver, S: spleen, Lu: Lung, and K: kidney). h The mean fluorescence intensity of different organs in each group (Mϕ, piezoMϕ, activated piezoMϕ) at different time points (n = 3 mice). i Schematic image of activated piezoMϕ with promoted cell migration in infected lung tissue. j Schematic diagram of cell migration Transwell test. k Transwell test cell staining (top: non-inflammatory condition, bottom: inflammatory condition, PC: 20% serum). l Statistical analysis results of Transwell test data (n = 5 independent samples). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001, ns denotes not significance. Data in (c–e, h, l) are presented as mean ± s.d. Statistical significances were assessed using two-tailed unpaired Student’s t-test (c, d, e) or two-way ANOVA with Sidak’s multiple comparisons test (h, l). Source data are provided as a Source Data file.