Fig. 3: ddPCR quantification of gene expression in tissues and localization within the nephron. | Nature Communications

Fig. 3: ddPCR quantification of gene expression in tissues and localization within the nephron.

From: Analysis of human urinary extracellular vesicles reveals disordered renal metabolism in myotonic dystrophy type 1

Fig. 3

a ddPCR quantification of PCK1, HPD, DPYS, GSTA1, ACY1, and ETFB normalized to reference gene FAM168A in human DM1 autopsy kidney tissue from individuals ages 50–55 with advanced DM1 (Muscle Impairment Rating Scale score of 4 [MIRS 4]26; N = 3) and age 80 with minimal DM1 (MIRS 3, including normal strength of ankle dorsiflexion; N = 1) and a CTG repeat expansion <100. Commercially available total RNA of human kidney served as a control. Error bars indicate ± s.e.m. b Renal tubule localization of Pck1 (black circles), Hpd (yellow squares), Dpys (purple triangles), Gsta1 (turquoise inverted triangles), Acy1 (orange diamonds), and Etfb (blue hexagons) expression by RNA sequencing of microdissected rat nephrons (Kidney Tubule Expression Atlas27). TPM transcripts per million, Prox. tubule proximal tubule, DCT distal convoluted tubule, Coll. duct collecting duct. Source data are provided as a Source Data file.

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