Fig. 5: RECQ4 exacerbates cellular function when challenged with other resolution pathways.

A Quantification of average bulky bridges per anaphase cell in HEK-293 (GEN1 -/-) and HEK-293 (wild-type) with siRNA as indicated. n = 3 independent experiments; data are means ± SD.; one-way ANOVA followed by Tukey’s multiple comparison test; p-value were adjusted by GraphPad Prism software; GEN1-WT/siControl vs GEN1-WT/siRecq4 **p = 0.0020; GEN1-WT/siControl vs GEN1-KO/siControl ***p = 0.0005; GEN1-WT/siControl vs GEN-KO/siRecq4 ****p < 0.0001; GEN1-WT/siRecq4 vs GEN1-KO/siControl ns p = 0.5461; GEN1-WT/siRecq4 vs GEN-KO/siRecq4 **p = 0.0034; GEN1-KO/siControl vs GEN-KO/siRecq4 *p = 0.0195. B Representative images of HEK-293 (GEN1 -/-) and HEK-293 (wild-type) cells treated with siRNA as indicated. Magnified images of anaphase cells displaying bulky bridges are shown. Scale bar = 5 µm. C Representative images of single and multiple UFBs. Scale bar = 5 µm. D Quantitative analysis of UFBs in U2OS cells (more than one UFB) with siRNA as indicated. n = 3 independent experiments; data are means ± SD.; one-way ANOVA followed by Tukey’s multiple comparison test; p-value were adjusted by GraphPad Prism software; siControl vs siRecq4 ns p = 0.0955; siControl vs siBLM ***p = 0.0007; siControl vs siRecq4/siBLM ****p < 0.0001; siRecq4 vs siBLM *p = 0.0195; siRecq4 vs siRecq4/siBLM ****p < 0.0001; siBLM vs sRecq4/siBLM **p = 0.0021. Source data are provided as a Source data file.