Fig. 4: ScRNA-seq analysis of Fu-Hp-treated PBMCs reveals a distinct macrophage-like cell cluster associated with inflammatory responses.
From: Fucosylated haptoglobin promotes inflammation via Mincle in sepsis: an observational study
a Unsupervised UMAP analysis on PBMCs pooled from three untreated and three Fu-Hp treated persons (16961 cells untreated, 14063 cells Fu-treated). Cell populations were mapped onto the UMAP landscape to visualize the distribution of cells across clusters using the RunUMAP and Dimplot function in seurat package. b Bar plot of the proportion of cell types shown in a, separated by 3 untreated samples and 3 Fu-Hp-treated samples. c The UMAP plot illustrating the alterations in cell proportions induced by Fu-Hp treatment. d Volcano plot depicting increased DEGs in Fu-Hp-treated PBMCs. Significantly increased cytokine/chemokine genes were labeled (adjusted p values < 0.06-301). P values were calculated by the FindMarkers function in Seurat 5.0.1 package using the default two-sided Wilcoxon Rank Sum test. e Ridge plots displaying the expression patterns of indicated genes in subpopulations of monocytes (2, 19, and 27) and macrophages (7, 18, and 29). f Expression of selected markers for sub-clusters (7, 18, and 29) of MLC populations.
