Fig. 3: Pre-NRC starvation prevents the mitochondrial import of Dib.
From: Nutrient status alters developmental fates via a switch in mitochondrial homeodynamics
A Colocalization of Dib-mCherry (red) and mitochondria (mito-GFP, green) were examined in PG cells from 72 h AEL to WPP. Right: Mander’s colocalization analyses. From left to right, n = 32, 60, 47, 40, 43 and 51, respectively. B Colocalization of Dib-mCherry (red) and mitochondria (mito-GFP, green) were examined in PG cells after 12 h, 24 h, and 36 h starvation from 72 h AEL. Right: Mander’s colocalization analyses. From left to right, n = 32, 54, 34, and 47, respectively. C, D Colocalization of Dib-mCherry (red) and mitochondria (C, mitotracker, green) or lysotracker (D green) were examined in PG cells of control and pre-NRC ST larvae at 108 h AEL. C n = 18 (108 h), 15 (pre-NRC ST). E, F Representative images of colocalization of Dib-mCherry (red) and mitochondria (mito-GFP, green) in PG cells (168 h AEL) with inhibition of PTTH, insulin, and TORC1 signalings E as well as mitochondria-associated genes and CCT genes F. E From left to right, n = 20, 21, 11, 31, 45, 21, 31, and 20, respectively. F From left to right, n = 25, 16, 13, 26, 32, 24, 16, 20, 20, 22, 23 and 23, respectively. G Representative images showing colocalization of Dib-mCherry (red) and mitochondria (mito-GFP, green) in PG cells (168 h AEL) with PG-specific expression of RasV12, Dp110CA, and Rheb. From left to right, n = 20, 61, 22 and 30, respectively. H, I Representative images showing colocalization of Dib-mCherry (red) and mitochondria (marked by mito-GFP or Tom20-GFP, green) in the PG (168 h AEL). Right: Mander’s colocalization analyses. From left to right, n = 30, 37, 9 and 25, respectively in (H, I). Two-tailed paired t test for two-column comparison. ***p < 0.001. J Developmental timing of phm > w1118 (n = 28) and phm > Marf (n = 15) under feeding conditions. K Body size of phm > w1118 and phm > Marf under feeding conditions. n = 58 (Phm > w1118), 51 (Phm > Marf). Two-tailed paired t test for two-column comparison. ***p < 0.001. All Data are presented as mean ± SD. Different lowercase letters represent statistical significance by one-way ANOVA. The genotypes and statistical data are provided in Supplementary Data 1.
