Fig. 3: Altered binding pose of SIRT7 in complex with the H3K18DTU nucleosome.

a Cryo-EM reconstruction showing SIRT7 bound to the H3K18DTU nucleosome. See Supplementary Figs. 9‒10 for data processing workflow, resolution and angular particle distribution. b Model of SIRT7(10-362) bound to the H3K18DTU nucleosome. See Supplementary Fig. 11 for model quality. SHL: superhelical location. c Comparison of SIRT7:nucleosome complexes bound to the H3K36 and H3K18 positions, with highlighted key differences. d Distance between residues that contact DNA for H3K36 binding and the DNA backbone in the H3K18-bound conformation. e LC-MS analysis of nucleosome deacetylation by SIRT7, showing the extracted ion chromatogram of the corresponding deacetylated product ([M + 21H]²¹⁺ ion). Nucleosome concentration was 200 nM. f LC-MS quantification of nucleosome deacylation by SIRT7 (50 nM concentration). Data represent the area under the curve (AUC, [M + 21H]²¹⁺ ion) of extracted ion chromatograms minus background. Error bars represent mean ± SD (n = 3 or 4) of distinct samples. Statistical analysis: unpaired t tests, two-tailed, ns: p > 0.05. Source data are provided as a Source Data file.