Table 1 Antibodies used for deacetylation assays with western blot readout
From: Structural basis of SIRT7 nucleosome engagement and substrate specificity
Target | Organism | Vendor | Cat. # | Dilution |
|---|---|---|---|---|
H3K18ac | Rabbit | ThermoFisher Scientific | 703896 | 1:1000 |
H3K36ac | Rabbit | Sigma-Aldrich | SAB5600229 | 1:1000 |
H3 (C-term) | Mouse | Cell Signaling Technology | 3638 | 1:2000 |
SIRT7 (N-term) | Rabbit | Cell Signaling Technology | 5360 | 1:1000 |
Mouse IgG (HRP-conjugated) | Horse | Cell Signaling Technology | 7076 | 1:10,000 |
Rabbit IgG (HRP-conjugated) | Goat | Cell Signaling Technology | 7074 | 1:10,000 |
