Table 3 Antibodies used for cell lysate western blots
From: Structural basis of SIRT7 nucleosome engagement and substrate specificity
Target | Organism | Vendor | Cat. # | Dilution |
|---|---|---|---|---|
SIRT7 (N-term) | Rabbit | Cell Signaling Technology | 5360 | 1:1000 |
mCherry | Rabbit | Proteintech | 26765-1-AP | 1:1000 |
GAPDH | Rabbit | Cell Signaling Technology | 2118 | 1:1000 |
Mouse IgG (HRP-conjugated) | Horse | Cell Signaling Technology | 7076 | 1:10,000 |
Rabbit IgG (HRP-conjugated) | Goat | Cell Signaling Technology | 7074 | 1:10,000 |
