Table 4 Antibodies used for immunofluorescence assays
From: Structural basis of SIRT7 nucleosome engagement and substrate specificity
Target | Organism | Vendor | Cat. # | Dilution | [Triton X-100] |
|---|---|---|---|---|---|
H3K18ac | Rabbit | ThermoFisher Scientific | MA5-24669 | 1:200 | 0.1% |
H3K36ac | Rabbit | ThermoFisher Scientific | MA5-24672 | 1:200 | - |
H4K16ac | Rabbit | ThermoFisher Scientific | MA5-34717 | 1:200 | 0.1% |
Rabbit IgG (Alexa Fluor 488-conjugated) | Donkey | ThermoFisher Scientific | A-21206 | 1:1000 or 1:300 | as above |
