Fig. 10: Kupffer cell-selective expression of LXRα W441F. | Nature Communications

Fig. 10: Kupffer cell-selective expression of LXRα W441F.

From: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis

Fig. 10

Male 11–12 week old Rosa26-LSL-FLAG-LXRα W441F/Lxrαfl/fl + Clec4f-Cre-tdTomato mice were fed the MASH diet for 4 weeks. a Experimental schematic. b Whole cell extracts were prepared from liver macrophage isolated from Cre–/– and Cre+/– mice, FLAG-tagged proteins were immune-purified and examined by Western blotting with anti-FLAG antibodies as described in the Methods. c, d Representative liver sections stained with antibodies recognizing Clec4f and Tim4. e–h Liver gene expression measured by real-time PCR as described in the Methods section. i, j Liver triglycerides and cholesterol were measured as described in the methods section. k, l Plasma ALT and AST measured as described in the Methods section. m–o Liver gene expression measured by real-time PCR as described in the Methods section. *Statistically significant difference between Cre–/– (n = 10) and Cre+/– (n = 7) mice determined by two-tailed Mann–Whitney tests (p ≤ 0.05). Boxes extend from the 25th–75th percentiles. The center line in the middle of the boxes represents the median. Whiskers extend from the lowest to the highest point. Source data are provided as Source Data file.

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